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Structural analysis of leader peptide binding enables leader-free cyanobactin processing.


ABSTRACT: Regioselective modification of amino acids within the context of a peptide is common to a number of biosynthetic pathways, and many of the resulting products have potential as therapeutics. The ATP-dependent enzyme LynD heterocyclizes multiple cysteine residues to thiazolines within a peptide substrate. The enzyme requires the substrate to have a conserved N-terminal leader for full activity. Catalysis is almost insensitive to immediately flanking residues in the substrate, suggesting that recognition occurs distant from the active site. Nucleotide and peptide substrate co-complex structures of LynD reveal that the substrate leader peptide binds to and extends the ?-sheet of a conserved domain of LynD, whereas catalysis is accomplished in another conserved domain. The spatial segregation of catalysis from recognition combines seemingly contradictory properties of regioselectivity and promiscuity, and it appears to be a conserved strategy in other peptide-modifying enzymes. A variant of LynD that efficiently processes substrates without a leader peptide has been engineered.

SUBMITTER: Koehnke J 

PROVIDER: S-EPMC4512242 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Structural analysis of leader peptide binding enables leader-free cyanobactin processing.

Koehnke Jesko J   Mann Greg G   Bent Andrew F AF   Ludewig Hannes H   Shirran Sally S   Botting Catherine C   Lebl Tomas T   Houssen Wael W   Jaspars Marcel M   Naismith James H JH  

Nature chemical biology 20150622 8


Regioselective modification of amino acids within the context of a peptide is common to a number of biosynthetic pathways, and many of the resulting products have potential as therapeutics. The ATP-dependent enzyme LynD heterocyclizes multiple cysteine residues to thiazolines within a peptide substrate. The enzyme requires the substrate to have a conserved N-terminal leader for full activity. Catalysis is almost insensitive to immediately flanking residues in the substrate, suggesting that recog  ...[more]

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