Two complementary approaches for intracellular delivery of exogenous enzymes.
Ontology highlight
ABSTRACT:
SUBMITTER: Rust A
PROVIDER: S-EPMC4513551 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Two complementary approaches for intracellular delivery of exogenous enzymes.
Rust Aleksander A Hassan Hazirah H A HH Sedelnikova Svetlana S Niranjan Dhevahi D Hautbergue Guillaume G Abbas Shaymaa A SA Partridge Lynda L Rice David D Binz Thomas T Davletov Bazbek B
Scientific reports 20150724
Intracellular delivery of biologically active proteins remains a formidable challenge in biomedical research. Here we show that biomedically relevant enzymes can be delivered into cells using a new DNA transfection reagent, lipofectamine 3000, allowing assessment of their intracellular functions. We also show that the J774.2 macrophage cell line exhibits unusual intracellular uptake of structurally and functionally distinct enzymes providing a convenient, reagent-free approach for evaluation of ...[more]
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