A Screen for Epigenetically Silenced microRNA Genes in Gastrointestinal Stromal Tumors.

ABSTRACT:

Background

Dysregulation of microRNA (miRNA) has been implicated in gastrointestinal stromal tumors (GISTs) but the mechanism is not fully understood. In this study, we aimed to explore the involvement of epigenetic alteration of miRNA genes in GISTs.

Methods

GIST-T1 cells were treated with 5-aza-2'-deoxycytidine (5-aza-dC) and 4-phenylbutyric acid (PBA), after which miRNA expression profiles were analyzed using TaqMan miRNA arrays. DNA methylation was then analyzed using bisulfite pyrosequencing. The functions of miRNAs were examined using MTT assays, wound-healing assays, Boyden chamber assays and Matrigel invasion assays. Gene expression microarrays were analyzed to assess effect of ectopic miRNA expression in GIST-T1 cells.

Results

Of the 754 miRNAs analyzed, 61 w

SUBMITTER: Isosaka M 

PROVIDER: S-EPMC4516245 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature