Ontology highlight
ABSTRACT:
SUBMITTER: Ruas M
PROVIDER: S-EPMC4516428 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Ruas Margarida M Davis Lianne C LC Chen Cheng-Chang CC Morgan Anthony J AJ Chuang Kai-Ting KT Walseth Timothy F TF Grimm Christian C Garnham Clive C Powell Trevor T Platt Nick N Platt Frances M FM Biel Martin M Wahl-Schott Christian C Parrington John J Galione Antony A
The EMBO journal 20150414 13
The second messenger NAADP triggers Ca(2+) release from endo-lysosomes. Although two-pore channels (TPCs) have been proposed to be regulated by NAADP, recent studies have challenged this. By generating the first mouse line with demonstrable absence of both Tpcn1 and Tpcn2 expression (Tpcn1/2(-/-)), we show that the loss of endogenous TPCs abolished NAADP-dependent Ca(2+) responses as assessed by single-cell Ca(2+) imaging or patch-clamp of single endo-lysosomes. In contrast, currents stimulated ...[more]