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MicroRNA profiling of clear cell renal cell cancer identifies a robust signature to define renal malignancy.


ABSTRACT: MicroRNAs are short single-stranded RNAs that are associated with gene regulation at the transcriptional and translational level. Changes in their expression were found in a variety of human cancers. Only few data are available on microRNAs in clear cell renal cell carcinoma (ccRCC). We performed genome-wide expression profiling of microRNAs using microarray analysis and quantification of specific microRNAs by TaqMan real-time RT-PCR. Matched malignant and non-malignant tissue samples from two independent sets of 12 and 72 ccRCC were profiled. The microarray-based experiments identified 13 over-expressed and 20 down-regulated microRNAs in malignant samples. Expression in ccRCC tissue samples compared with matched non-malignant samples measured by RT-PCR was increased on average by 2.7- to 23-fold for the hsa-miR-16, -452*, -224, -155 and -210, but decreased by 4.8- to 138-fold for hsa-miR-200b, -363, -429, -200c, -514 and -141. No significant associations between these differentially expressed microRNAs and the clinico-pathological factors tumour stage, tumour grade and survival rate were found. Nevertheless, malignant and non-malignant tissue could clearly be differentiated by their microRNA profile. A combination of miR-141 and miR-155 resulted in a 97% overall correct classification of samples. The presented differential microRNA pattern provides a solid basis for further validation, including functional studies.

SUBMITTER: Jung M 

PROVIDER: S-EPMC4516539 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature

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MicroRNA profiling of clear cell renal cell cancer identifies a robust signature to define renal malignancy.

Jung Monika M   Mollenkopf Hans-Joachim HJ   Grimm Christina C   Wagner Ina I   Albrecht Marco M   Waller Tobias T   Pilarsky Christian C   Johannsen Manfred M   Stephan Carsten C   Lehrach Hans H   Nietfeld Wilfried W   Rudel Thomas T   Jung Klaus K   Kristiansen Glen G  

Journal of cellular and molecular medicine 20090217 9B


MicroRNAs are short single-stranded RNAs that are associated with gene regulation at the transcriptional and translational level. Changes in their expression were found in a variety of human cancers. Only few data are available on microRNAs in clear cell renal cell carcinoma (ccRCC). We performed genome-wide expression profiling of microRNAs using microarray analysis and quantification of specific microRNAs by TaqMan real-time RT-PCR. Matched malignant and non-malignant tissue samples from two i  ...[more]

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