Ontology highlight
ABSTRACT:
SUBMITTER: Chao J
PROVIDER: S-EPMC4517909 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Chao Jerry J Lee Taiyoon T Ward E Sally ES Ober Raimund J RJ
PloS one 20150728 7
The localization of fluorescent microspheres is often employed for drift correction and image registration in single molecule microscopy, and is commonly carried out by fitting a point spread function to the image of the given microsphere. The mismatch between the point spread function and the image of the microsphere, however, calls into question the suitability of this localization approach. To investigate this issue, we subject both simulated and experimental microsphere image data to a maxim ...[more]