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Single-cell magnetic imaging using a quantum diamond microscope.

ABSTRACT: We apply a quantum diamond microscope for detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and a field of view (?1 mm(2)) two orders of magnitude larger than that of previous NV imaging technologies, enabling practical applications. To illustrate, we quantified cancer biomarkers expressed by rare tumor cells in a large population of healthy cells.

SUBMITTER: Glenn DR

PROVIDER: S-EPMC4521973 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Single-cell magnetic imaging using a quantum diamond microscope.

Glenn D R DR Lee K K Park H H Weissleder R R Yacoby A A Lukin M D MD Lee H H Walsworth R L RL Connolly C B CB

Nature methods 20150622 8

We apply a quantum diamond microscope for detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and a field of view (∼1 mm(2)) two orders of magnitude larger than that of previous NV imaging technologies, enabling practical applications. To illustrate, we quantified cancer biomarkers expressed by rare tumor cells in a large population of ...[more]

PMID: 26098019

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