Ontology highlight
ABSTRACT:
SUBMITTER: Sardar D
PROVIDER: S-EPMC4522240 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Sardar Debosmita D Lin Zhenjian Z Schmidt Eric W EW
Chemistry & biology 20150709 7
Macrocyclases and other posttranslational enzymes afford derived peptides with improved properties for pharmaceutical and biotechnological applications. Here, we asked whether multiple posttranslational modifications could be simultaneously controlled and matched to rationally generate new peptide derivatives. We reconstituted the cyanobactin peptide natural products in vitro with up to five different posttranslational enzymes in a single tube. By manipulating the order of addition and identity ...[more]