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Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression.

ABSTRACT: Although mRNA has several advantages over plasmid DNA when delivered into cells for gene expression, mRNA transfection is a very rare occurrence in gene delivery. This is mainly because of the labile nature of RNA, resulting in a low expression level of the desired protein. In this study, self-assembled mRNA nanoparticles (mRNA-NPs) packed with multiple repeats of mRNA were synthesized to achieve efficient gene expression. This approach required only a one-step process to synthesize particles with a minimal amount of plasmid DNA to produce the RNA transcripts via rolling circle transcription. Moreover, there are no concerns for cytotoxicity which can be caused by chemical condensates because mRNA-NPs are made entirely of mRNA. An examination of the cells transfected with the mRNA-NPs encoding the green fluorescence protein (GFP) confirmed that the mRNA-NPs can be used as a novel platform for effective gene delivery.

SUBMITTER: Kim H

PROVIDER: S-EPMC4522648 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression.

Kim Hyejin H Park Yongkuk Y Lee Jong Bum JB

Scientific reports 20150803

Although mRNA has several advantages over plasmid DNA when delivered into cells for gene expression, mRNA transfection is a very rare occurrence in gene delivery. This is mainly because of the labile nature of RNA, resulting in a low expression level of the desired protein. In this study, self-assembled mRNA nanoparticles (mRNA-NPs) packed with multiple repeats of mRNA were synthesized to achieve efficient gene expression. This approach required only a one-step process to synthesize particles wi ...[more]

PMID: 26235529

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