Unknown

Dataset Information

0

Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression.


ABSTRACT: Although mRNA has several advantages over plasmid DNA when delivered into cells for gene expression, mRNA transfection is a very rare occurrence in gene delivery. This is mainly because of the labile nature of RNA, resulting in a low expression level of the desired protein. In this study, self-assembled mRNA nanoparticles (mRNA-NPs) packed with multiple repeats of mRNA were synthesized to achieve efficient gene expression. This approach required only a one-step process to synthesize particles with a minimal amount of plasmid DNA to produce the RNA transcripts via rolling circle transcription. Moreover, there are no concerns for cytotoxicity which can be caused by chemical condensates because mRNA-NPs are made entirely of mRNA. An examination of the cells transfected with the mRNA-NPs encoding the green fluorescence protein (GFP) confirmed that the mRNA-NPs can be used as a novel platform for effective gene delivery.

SUBMITTER: Kim H 

PROVIDER: S-EPMC4522648 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression.

Kim Hyejin H   Park Yongkuk Y   Lee Jong Bum JB  

Scientific reports 20150803


Although mRNA has several advantages over plasmid DNA when delivered into cells for gene expression, mRNA transfection is a very rare occurrence in gene delivery. This is mainly because of the labile nature of RNA, resulting in a low expression level of the desired protein. In this study, self-assembled mRNA nanoparticles (mRNA-NPs) packed with multiple repeats of mRNA were synthesized to achieve efficient gene expression. This approach required only a one-step process to synthesize particles wi  ...[more]

Similar Datasets

| S-EPMC3965374 | biostudies-literature
| S-EPMC4046771 | biostudies-literature
| S-EPMC7837307 | biostudies-literature
| S-EPMC9737671 | biostudies-literature
| S-EPMC8578456 | biostudies-literature
| S-EPMC8341208 | biostudies-literature
| S-EPMC9751278 | biostudies-literature
| S-EPMC6732788 | biostudies-literature
| S-EPMC8390897 | biostudies-literature
| S-EPMC8733341 | biostudies-literature