Label-free single-cell protein quantification using a drop-based mix-and-read system.
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ABSTRACT:
SUBMITTER: Abbaspourrad A
PROVIDER: S-EPMC4522677 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Publications
Label-free single-cell protein quantification using a drop-based mix-and-read system.
Abbaspourrad Alireza A Zhang Huidan H Tao Ye Y Cui Naiwen N Asahara Haruichi H Zhou Ying Y Yue Dongxian D Koehler Stephan A SA Ung Lloyd W LW Heyman John J Ren Yukun Y Ziblat Roy R Chong Shaorong S Weitz David A DA
Scientific reports 20150803
Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry. ...[more]
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