Ontology highlight
ABSTRACT:
SUBMITTER: Holmes JB
PROVIDER: S-EPMC4522766 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Holmes J Bradley JB Akman Gokhan G Wood Stuart R SR Sakhuja Kiran K Cerritelli Susana M SM Moss Chloe C Bowmaker Mark R MR Jacobs Howard T HT Crouch Robert J RJ Holt Ian J IJ
Proceedings of the National Academy of Sciences of the United States of America 20150710 30
Encoding ribonuclease H1 (RNase H1) degrades RNA hybridized to DNA, and its function is essential for mitochondrial DNA maintenance in the developing mouse. Here we define the role of RNase H1 in mitochondrial DNA replication. Analysis of replicating mitochondrial DNA in embryonic fibroblasts lacking RNase H1 reveals retention of three primers in the major noncoding region (NCR) and one at the prominent lagging-strand initiation site termed Ori-L. Primer retention does not lead immediately to de ...[more]