Dataset Information

Simvastatin alleviates cardiac fibrosis induced by infarction via up-regulation of TGF-? receptor III expression.

ABSTRACT:

Background and purpose

Statins decrease heart disease risk, but their mechanisms are not completely understood. We examined the role of the TGF-? receptor III (TGFBR3) in the inhibition of cardiac fibrosis by simvastatin.

Experimental approach

Myocardial infarction (MI) was induced by ligation of the left anterior descending coronary artery in mice given simvastatin orally for 7 days. Cardiac fibrosis was measured by Masson staining and electron microscopy. Heart function was evaluated by echocardiography. Signalling through TGFBR3, ERK1/2, JNK and p38 pathways was measured using Western blotting. Collagen content and cell viability were measured in cultures of neonatal mouse cardiac fibroblasts (NMCFs). Interactions between TGFBR3 and the scaffolding protein, GAIP-interacting protein C-terminus (GIPC) were detected using co-immunoprecipitation (co-IP). In vivo, hearts were injected with lentivirus carrying shRNA for TGFBR3.

Key results

Simvastatin prevented fibrosis following MI, improved heart ultrastructure and function, up-regulated TGFBR3 and decreased ERK1/2 and JNK phosphorylation. Simvastatin up-regulated TGFBR3 in NMCFs, whereas silencing TGFBR3 reversed inhibitory effects of simvastatin on cell proliferation and collagen production. Simvastatin inhibited ERK1/2 and JNK signalling while silencing TGFBR3 opposed this effect. Co-IP demonstrated TGFBR3 binding to GIPC. Overexpressing TGFBR3 inhibited ERK1/2 and JNK signalling which was abolished by knock-down of GIPC. In vivo, suppression of cardiac TGFBR3 abolished anti-fibrotic effects, improvement of cardiac function and changes in related proteins after simvastatin.

Conclusions and implications

TGFBR3 mediated the decreased cardiac fibrosis, collagen deposition and fibroblast activity, induced by simvastatin, following MI. These effects involved GIPC inhibition of the ERK1/2/JNK pathway.

SUBMITTER: Sun F

PROVIDER: S-EPMC4523335 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Publications

Simvastatin alleviates cardiac fibrosis induced by infarction via up-regulation of TGF-β receptor III expression.

Sun Fei F Duan Wenqi W Zhang Yu Y Zhang Lingling L Qile Muge M Liu Zengyan Z Qiu Fang F Zhao Dan D Lu Yanjie Y Chu Wenfeng W

British journal of pharmacology 20150612 15

<h4>Background and purpose</h4>Statins decrease heart disease risk, but their mechanisms are not completely understood. We examined the role of the TGF-β receptor III (TGFBR3) in the inhibition of cardiac fibrosis by simvastatin.<h4>Experimental approach</h4>Myocardial infarction (MI) was induced by ligation of the left anterior descending coronary artery in mice given simvastatin orally for 7 days. Cardiac fibrosis was measured by Masson staining and electron microscopy. Heart function was eval ...[more]

PMID: 25884615

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