Unknown

Dataset Information

0

Characterization of Ribosomal Frameshifting in Theiler's Murine Encephalomyelitis Virus.


ABSTRACT: Theiler's murine encephalomyelitis virus (TMEV) is a member of the genus Cardiovirus in the Picornaviridae, a family of positive-sense single-stranded RNA viruses. Previously, we demonstrated that in the related cardiovirus, Encephalomyocarditis virus, a programmed-1 ribosomal frameshift (1 PRF) occurs at a conserved G_GUU_UUU sequence within the 2B-encoding region of the polyprotein open reading frame (ORF). Here we show that-1 PRF occurs at a similar site during translation of the TMEV genome. In addition, we demonstrate that a predicted 3= RNA stem-loop structure at a noncanonical spacing downstream of the shift site is required for efficient frameshifting in TMEV and that frameshifting also requires virus infection. Mutating the G_GUU_UUU shift site to inhibit frameshifting results in an attenuated virus with reduced growth kinetics and a small-plaque phenotype. Frameshifting in the virus context was found to be extremely efficient at 74 to 82%, which, to our knowledge, is the highest frameshifting efficiency recorded to date for any virus. We propose that highly efficient-1 PRF in TMEV provides a mechanism to escape the confines of equimolar expression normally inherent in the single-polyprotein expression strategy of picornaviruses.

SUBMITTER: Finch LK 

PROVIDER: S-EPMC4524249 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterization of Ribosomal Frameshifting in Theiler's Murine Encephalomyelitis Virus.

Finch Leanne K LK   Ling Roger R   Napthine Sawsan S   Olspert Allan A   Michiels Thomas T   Lardinois Cécile C   Bell Susanne S   Loughran Gary G   Brierley Ian I   Firth Andew E AE  

Journal of virology 20150801 16


Theiler's murine encephalomyelitis virus (TMEV) is a member of the genus Cardiovirus in the Picornaviridae, a family of positive-sense single-stranded RNA viruses. Previously, we demonstrated that in the related cardiovirus, Encephalomyocarditis virus, a programmed-1 ribosomal frameshift (1 PRF) occurs at a conserved G_GUU_UUU sequence within the 2B-encoding region of the polyprotein open reading frame (ORF). Here we show that-1 PRF occurs at a similar site during translation of the TMEV genome.  ...[more]

Similar Datasets

| S-EPMC6735917 | biostudies-literature
| S-EPMC3126553 | biostudies-literature
| S-EPMC6390522 | biostudies-literature
| S-EPMC2528868 | biostudies-literature
| S-EPMC3335973 | biostudies-literature
| S-EPMC104082 | biostudies-literature
| S-EPMC8886069 | biostudies-literature
| S-EPMC6834305 | biostudies-literature
| S-EPMC8029129 | biostudies-literature
| S-EPMC10434537 | biostudies-literature