Ontology highlight
ABSTRACT:
SUBMITTER: Srivastava P
PROVIDER: S-EPMC4525152 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Srivastava Poonam P Yang Hao H Ellis-Guardiola Ken K Lewis Jared C JC
Nature communications 20150724
Artificial metalloenzymes (ArMs) formed by incorporating synthetic metal catalysts into protein scaffolds have the potential to impart to chemical reactions selectivity that would be difficult to achieve using metal catalysts alone. In this work, we covalently link an alkyne-substituted dirhodium catalyst to a prolyl oligopeptidase containing a genetically encoded L-4-azidophenylalanine residue to create an ArM that catalyses olefin cyclopropanation. Scaffold mutagenesis is then used to improve ...[more]