Upregulation of nemo-like kinase is an independent prognostic factor in colorectal cancer.

ABSTRACT:

Aim

To investigate the expression and oncogenic role of nemo-like kinase (NLK) in colorectal cancer.

Methods

Expression of NLK protein was assessed by immunohistochemistry in tissue specimens from 56 cases of normal colorectal mucosa, 51 cases of colorectal adenoma, and 712 cases of colorectal cancer. In addition, NLK expression was knocked down using a lentivirus carrying NLK small hairpin RNA in colorectal cancer cells. Cell viability methylthiazoletetrazolium assays, colony formation assays, flow cytometry cell cycle assays, Transwell migration assays, and gene expression assays were performed to explore its role on proliferation and migration of colorectal cancer.

Results

Expression of NLK protein progressively increased in tissues from the normal mucosa through a

SUBMITTER: Zhang W 

PROVIDER: S-EPMC4528026 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature