Ontology highlight
ABSTRACT:
SUBMITTER: Whiteaker JR
PROVIDER: S-EPMC4528251 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Whiteaker Jeffrey R JR Zhao Lei L Yan Ping P Ivey Richard G RG Voytovich Uliana J UJ Moore Heather D HD Lin Chenwei C Paulovich Amanda G AG
Molecular & cellular proteomics : MCP 20150518 8
In most cell signaling experiments, analytes are measured one Western blot lane at a time in a semiquantitative and often poorly specific manner, limiting our understanding of network biology and hindering the translation of novel therapeutics and diagnostics. We show the feasibility of using multiplex immuno-MRM for phospho-pharmacodynamic measurements, establishing the potential for rapid and precise quantification of cell signaling networks. A 69-plex immuno-MRM assay targeting the DNA damage ...[more]