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Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.

ABSTRACT: Monodisperse alginate microgels (10-50 ?m) are created via droplet-based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.

SUBMITTER: Utech S

PROVIDER: S-EPMC4529809 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.

Utech Stefanie S Prodanovic Radivoje R Mao Angelo S AS Ostafe Raluca R Mooney David J DJ Weitz David A DA

Advanced healthcare materials 20150603 11

Monodisperse alginate microgels (10-50 μm) are created via droplet-based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments. ...[more]

PMID: 26039892

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