Ontology highlight
ABSTRACT:
SUBMITTER: Llacer JL
PROVIDER: S-EPMC4534855 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Llácer Jose L JL Hussain Tanweer T Marler Laura L Aitken Colin Echeverría CE Thakur Anil A Lorsch Jon R JR Hinnebusch Alan G AG Ramakrishnan V V
Molecular cell 20150723 3
Translation initiation in eukaryotes begins with the formation of a pre-initiation complex (PIC) containing the 40S ribosomal subunit, eIF1, eIF1A, eIF3, ternary complex (eIF2-GTP-Met-tRNAi), and eIF5. The PIC, in an open conformation, attaches to the 5' end of the mRNA and scans to locate the start codon, whereupon it closes to arrest scanning. We present single particle cryo-electron microscopy (cryo-EM) reconstructions of 48S PICs from yeast in these open and closed states, at 6.0 Å and 4.9 Å ...[more]