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First report of bacterial community from a Bat Guano using Illumina next-generation sequencing.


ABSTRACT: V4 hypervariable region of 16S rDNA was analyzed for identifying the bacterial communities present in Bat Guano from the unexplored cave - Pnahkyndeng, Meghalaya, Northeast India. Metagenome comprised of 585,434 raw Illumina sequences with a 59.59% G+C content. A total of 416,490 preprocessed reads were clustered into 1282 OTUs (operational taxonomical units) comprising of 18 bacterial phyla. The taxonomic profile showed that the guano bacterial community is dominated by Chloroflexi, Actinobacteria and Crenarchaeota which account for 70.73% of all sequence reads and 43.83% of all OTUs. Metagenome sequence data are available at NCBI under the accession no. SRP051094. This study is the first to characterize Bat Guano bacterial community using next-generation sequencing approach.

SUBMITTER: De Mandal S 

PROVIDER: S-EPMC4535889 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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First report of bacterial community from a Bat Guano using Illumina next-generation sequencing.

De Mandal Surajit S   Zothansanga   Panda Amritha Kumari AK   Bisht Satpal Singh SS   Senthil Kumar Nachimuthu N  

Genomics data 20150409


V4 hypervariable region of 16S rDNA was analyzed for identifying the bacterial communities present in Bat Guano from the unexplored cave - Pnahkyndeng, Meghalaya, Northeast India. Metagenome comprised of 585,434 raw Illumina sequences with a 59.59% G+C content. A total of 416,490 preprocessed reads were clustered into 1282 OTUs (operational taxonomical units) comprising of 18 bacterial phyla. The taxonomic profile showed that the guano bacterial community is dominated by Chloroflexi, Actinobacte  ...[more]

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