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High-yield isolation of extracellular vesicles using aqueous two-phase system.

ABSTRACT: Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.

SUBMITTER: Shin H

PROVIDER: S-EPMC4536486 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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High-yield isolation of extracellular vesicles using aqueous two-phase system.

Shin Hyunwoo H Han Chungmin C Labuz Joseph M JM Kim Jiyoon J Kim Jongmin J Cho Siwoo S Gho Yong Song YS Takayama Shuichi S Park Jaesung J

Scientific reports 20150814

Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). Thi ...[more]

PMID: 26271727

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