Ontology highlight
ABSTRACT:
SUBMITTER: Preston Campbell J
PROVIDER: S-EPMC4536516 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Scientific reports 20150814
Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization i ...[more]