Photodynamic activation as a molecular switch to promote osteoblast cell differentiation via AP-1 activation.

ABSTRACT: In photodynamic therapy (PDT), cells are impregnated with a photosensitizing agent that is activated by light irradiation, thereby photochemically generating reactive oxygen species (ROS). The amounts of ROS produced depends on the PDT dose and the nature of the photosensitizer. Although high levels of ROS are cytotoxic, at physiological levels they play a key role as second messengers in cellular signaling pathways, pluripotency, and differentiation of stem cells. To investigate further the use of photochemically triggered manipulation of such pathways, we exposed mouse osteoblast precursor cells and rat primary mesenchymal stromal cells to low-dose PDT. Our results demonstrate that low-dose PDT can promote osteoblast differentiation via the activation of activator protein-1 (AP-1). Altho

SUBMITTER: Kushibiki T 

PROVIDER: S-EPMC4538568 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature