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Mitochondria-targeted antiaging gene therapy with adeno-associated viral vectors.

ABSTRACT: Transgenic expression of catalase in mitochondria using a transgenic strategy extends life span and prevents aging-related pathology in mice. However, transgenic overexpression is not suitable for a clinical application. Adeno-associated virus (AAV) is the most promising gene delivery vehicle. Here we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT on muscle contractility and running performance in mice.

SUBMITTER: Li D 

PROVIDER: S-EPMC4539949 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Mitochondria-targeted antiaging gene therapy with adeno-associated viral vectors.

Li Dejia D   Duan Dongsheng D  

Methods in molecular biology (Clifton, N.J.) 20130101

Transgenic expression of catalase in mitochondria using a transgenic strategy extends life span and prevents aging-related pathology in mice. However, transgenic overexpression is not suitable for a clinical application. Adeno-associated virus (AAV) is the most promising gene delivery vehicle. Here we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT o  ...[more]

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