Unknown

Dataset Information

0

Identification and characterization of two ankyrin-B isoforms in mammalian heart.


ABSTRACT: Excitation-contraction coupling in cardiomyocytes requires the proper targeting and retention of membrane proteins to unique domains by adaptor proteins like ankyrin-B. While ankyrin-B has been shown to interact with a variety of membrane and structural proteins located at different subcellular domains in cardiomyocytes, what regulates the specificity of ankyrin-B for particular interacting proteins remains elusive.Here, we report the identification of two novel ankyrin-B isoforms AnkB-188 and AnkB-212 in human, rat, and mouse hearts. Novel cDNAs for both isoforms were isolated by long-range PCR of reverse-transcribed mRNA isolated from human ventricular tissue. The isoforms can be discriminated based on their function and subcellular distribution in cardiomyocytes. Heterologous overexpression of AnkB-188 increases sodium-calcium exchanger (NCX) membrane expression and current, while selective knockdown of AnkB-188 in cardiomyocytes reduces NCX expression and localization in addition to causing irregular contraction rhythms. Using an isoform-specific antibody, we demonstrate that the expression of AnkB-212 is restricted to striated muscles and is localized to the M-line of cardiomyocytes by interacting with obscurin. Selective knockdown of AnkB-212 significantly attenuates the expression of endogenous ankyrin-B at the M-line but does not disrupt NCX expression at transverse tubules in cardiomyocytes.The identification and characterization of two functionally distinct ankyrin-B isoforms in heart provide compelling evidence that alternative splicing of the ANK2 gene regulates the fidelity of ankyrin-B interactions with proteins.

SUBMITTER: Wu HC 

PROVIDER: S-EPMC4540146 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Identification and characterization of two ankyrin-B isoforms in mammalian heart.

Wu Henry C HC   Yamankurt Gokay G   Luo JiaLie J   Subramaniam Janani J   Hashmi Syed Shahrukh SS   Hu Hongzhen H   Cunha Shane R SR  

Cardiovascular research 20150624 4


<h4>Aims</h4>Excitation-contraction coupling in cardiomyocytes requires the proper targeting and retention of membrane proteins to unique domains by adaptor proteins like ankyrin-B. While ankyrin-B has been shown to interact with a variety of membrane and structural proteins located at different subcellular domains in cardiomyocytes, what regulates the specificity of ankyrin-B for particular interacting proteins remains elusive.<h4>Methods and results</h4>Here, we report the identification of tw  ...[more]

Similar Datasets

| S-EPMC3606321 | biostudies-literature
| S-EPMC2910051 | biostudies-literature
| S-EPMC5293009 | biostudies-literature
| S-EPMC2868064 | biostudies-literature
| S-EPMC2040269 | biostudies-literature
| S-EPMC3436279 | biostudies-literature
| S-EPMC3000613 | biostudies-literature
| S-EPMC2802713 | biostudies-literature
| S-EPMC5352492 | biostudies-literature
| S-EPMC102140 | biostudies-literature