Ontology highlight
ABSTRACT:
SUBMITTER: Stevanato L
PROVIDER: S-EPMC4541566 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Stevanato Lara L Hicks Caroline C Sinden John D JD
Journal of visualized experiments : JoVE 20150412 98
Neural stem cells (NSCs) are capable of self-renewal and differentiation into neurons, astrocytes and oligodendrocytes under specific local microenvironments. In here, we present a set of methods used for three dimensional (3D) differentiation and miRNA analysis of a clonal human neural stem cell (hNSC) line, currently in clinical trials for stroke disability (NCT01151124 and NCT02117635, Clinicaltrials.gov). HNSCs were derived from an ethical approved first trimester human fetal cortex and cond ...[more]