Ontology highlight
ABSTRACT:
SUBMITTER: Farlik M
PROVIDER: S-EPMC4542311 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Farlik Matthias M Sheffield Nathan C NC Nuzzo Angelo A Datlinger Paul P Schönegger Andreas A Klughammer Johanna J Bock Christoph C
Cell reports 20150226 8
Methods for single-cell genome and transcriptome sequencing have contributed to our understanding of cellular heterogeneity, whereas methods for single-cell epigenomics are much less established. Here, we describe a whole-genome bisulfite sequencing (WGBS) assay that enables DNA methylation mapping in very small cell populations (μWGBS) and single cells (scWGBS). Our assay is optimized for profiling many samples at low coverage, and we describe a bioinformatic method that analyzes collections of ...[more]