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Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping.

ABSTRACT: We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca(2+) level at the individual-cell level.

SUBMITTER: Yoon J

PROVIDER: S-EPMC4543936 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping.

Yoon Jonghee J Lee Minji M Lee KyeoReh K Kim Nury N Kim Jin Man JM Park Jongchan J Yu Hyeonseung H Choi Chulhee C Heo Won Do WD Park YongKeun Y

Scientific reports 20150821

We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca(2+) level at the individual-cell level. ...[more]

PMID: 26293590

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