A Clickable Aminooxy Probe for Monitoring Cellular ADP-Ribosylation.
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ABSTRACT:
SUBMITTER: Morgan RK
PROVIDER: S-EPMC4546562 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Publications
A Clickable Aminooxy Probe for Monitoring Cellular ADP-Ribosylation.
Morgan Rory K RK Cohen Michael S MS
ACS chemical biology 20150527 8
ADP-ribosylation is essential for cell function, yet there is a dearth of methods for detecting this post-translational modification in cells. Here, we describe a clickable aminooxy alkyne (AO-alkyne) probe that can detect cellular ADP-ribosylation on acidic amino acids following Cu-catalyzed conjugation to an azide-containing reporter. Using AO-alkyne, we show that PARP10 and PARP11 are auto-ADP-ribosylated in cells. We also demonstrate that AO-alkyne can be used to monitor stimulus-induced ADP ...[more]
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