Characterization of circulating transfer RNA-derived RNA fragments in cattle.

ABSTRACT: The objective was to characterize naturally occurring circulating transfer RNA-derived RNA fragments (tRFs) in cattle. Serum from eight clinically normal adult dairy cows was collected, and small non-coding RNAs were extracted immediately after collection and sequenced by Illumina MiSeq. Sequences aligned to transfer RNA (tRNA) genes or their flanking sequences were characterized. Sequences aligned to the beginning of 5' end of the mature tRNA were classified as tRF5; those aligned to the 3' end of mature tRNA were classified as tRF3; and those aligned to the beginning of the 3' end flanking sequences were classified as tRF1. There were 3,190,962 sequences that mapped to transfer RNA and small non-coding RNAs in the bovine genome. Of these, 2,323,520 were identified as tRF5s, 562 were tRF3

SUBMITTER: Casas E 

PROVIDER: S-EPMC4547532 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature