Ontology highlight
ABSTRACT:
SUBMITTER: Chande AG
PROVIDER: S-EPMC4548221 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Chande Ajit G AG Siddiqui Zaved Z Midha Mukul Kumar MK Sirohi Varsha V Ravichandran Srikanth S Rao Kanury V S KV
Scientific reports 20150825
Upon infection, Mycobacterium tuberculosis (Mtb) deploys specialized secretion machinery to deliver virulent proteins with the capacity to modulate a variety of host-cellular pathways. Studies on the identification of intra-macrophage Mtb proteins, however, are constricted by an inability to selectively enrich these virulent effectors against overwhelming protein content of the host. Here, we introduce an Mtb-selective protein labeling method based on genetic incorporation of azidonorleucine (An ...[more]