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Reconfigurable hybrid interface for molecular marker diagnostics and in-situ reporting.

ABSTRACT: Combinations of molecular signals such as transcription factors and microRNAs in cells are a reliable indicator of multi-gene disorders. A system capable of detecting these conditions in-situ may be used as a tool for diagnosis and monitoring of disease. Here, we engineer genetic circuits that sense endogenous levels of the androgen receptor (AR), the glucocorticoid receptor (GR), and the microRNA hsa-miR-21 (miR-21) in cervical cancer cells (HeLa). Furthermore, using the mediator molecule human chorionic gonadotropin (hCG), we interface the intracellular information to enzyme-linked immunosorbent assay (ELISA) test strips. We demonstrate that this hybrid genetic circuit and test-strip interface can accommodate combinatorial, low-cost, and in-situ reporting, a versatile profiling tool.

SUBMITTER: Ehrhardt K

PROVIDER: S-EPMC4549190 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Reconfigurable hybrid interface for molecular marker diagnostics and in-situ reporting.

Ehrhardt Kristina K Guinn Michael T MT Quarton Tyler T Zhang Michael Q MQ Bleris Leonidas L

Biosensors & bioelectronics 20150717

Combinations of molecular signals such as transcription factors and microRNAs in cells are a reliable indicator of multi-gene disorders. A system capable of detecting these conditions in-situ may be used as a tool for diagnosis and monitoring of disease. Here, we engineer genetic circuits that sense endogenous levels of the androgen receptor (AR), the glucocorticoid receptor (GR), and the microRNA hsa-miR-21 (miR-21) in cervical cancer cells (HeLa). Furthermore, using the mediator molecule human ...[more]

PMID: 26210472

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