Unknown

Dataset Information

0

Arabidopsis PCNAs form complexes with selected D-type cyclins.


ABSTRACT: Proliferating Cell Nuclear Antigen (PCNA) is a key nuclear protein of eukaryotic cells. It has been shown to form complexes with cyclin dependent kinases, cyclin dependent kinase inhibitors and the D-type cyclins which are involved in the cell cycle control. In Arabidopsis two genes coding for PCNA1 and PCNA2 proteins have been identified. In this study by analyzing Arabidopsis PCNA/CycD complexes we tested the possible functional differentiation of PCNA1/2 proteins in cell cycle control. Most out of the 10 cyclins investigated showed only nuclear localization except CycD2;1, CycD4;1, and CycD4;2 which were observed both in the nucleus and cytoplasm. Using the Y2H, BiFC and FLIM-FRET techniques we identified D-type cyclins which formed complexes with either PCNA1 or PCNA2. Among the candidates tested only CycD1;1, CycD3;1, and CycD3;3 were not detected in a complex with the PCNA proteins. Moreover, our results indicate that the formation of CycD3;2/PCNA and CycD4;1/PCNA complexes can be regulated by other as yet unidentified factor(s). Additionally, FLIM-FRET analyses suggested that in planta the distance between PCNA1/CycD4;1, PCNA1/CycD6;1, PCNA1/CycD7;1, and PCNA2/CycD4;2 proteins was shorter than that between PCNA2/CycD4;1, PCNA2/CycD6;1, PCNA2/CycD7;1, and PCNA1/CycD4;2 pairs. These data indicate that the nine amino acid differences between PCNA1 and PCNA2 have an impact on the architecture of Arabidopsis CycD/PCNA complexes.

SUBMITTER: Strzalka WK 

PROVIDER: S-EPMC4550699 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

Arabidopsis PCNAs form complexes with selected D-type cyclins.

Strzalka Wojciech K WK   Aggarwal Chhavi C   Krzeszowiec Weronika W   Jakubowska Agata A   Sztatelman Olga O   Banas Agnieszka K AK  

Frontiers in plant science 20150717


Proliferating Cell Nuclear Antigen (PCNA) is a key nuclear protein of eukaryotic cells. It has been shown to form complexes with cyclin dependent kinases, cyclin dependent kinase inhibitors and the D-type cyclins which are involved in the cell cycle control. In Arabidopsis two genes coding for PCNA1 and PCNA2 proteins have been identified. In this study by analyzing Arabidopsis PCNA/CycD complexes we tested the possible functional differentiation of PCNA1/2 proteins in cell cycle control. Most o  ...[more]

Similar Datasets

| S-EPMC8728612 | biostudies-literature
| S-EPMC3871821 | biostudies-other
| S-EPMC4507761 | biostudies-literature
| S-SCDT-EMBOR-2021-53995-T | biostudies-other
| S-EPMC1266134 | biostudies-literature
| S-EPMC3388828 | biostudies-other
| S-EPMC10378862 | biostudies-literature
| S-EPMC4036514 | biostudies-literature
| S-EPMC3276088 | biostudies-other
| S-EPMC3709509 | biostudies-literature