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High Throughput Screening Method to Explore Protein Interactions with Nanoparticles.

ABSTRACT: The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partners and conformational changes on time scales ranging from milliseconds to days. Mobile fluorophores are used as reporters to study the interaction between proteins and nanoparticles in a high-throughput manner in multi-well format. Furthermore, the screening method may reveal changes in colloidal stability of nanomaterials depending on the physicochemical conditions.

SUBMITTER: Nasir I

PROVIDER: S-EPMC4551901 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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High Throughput Screening Method to Explore Protein Interactions with Nanoparticles.

Nasir Irem I Fatih Warda W Svensson Anja A Radu Dennis D Linse Sara S Cabaleiro Lago Celia C Lundqvist Martin M

PloS one 20150827 8

The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partner ...[more]

PMID: 26313757

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Dataset Information

High Throughput Screening Method to Explore Protein Interactions with Nanoparticles.

Publications

High Throughput Screening Method to Explore Protein Interactions with Nanoparticles.

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