Unknown

Dataset Information

0

Transcriptional slippage in the positive-sense RNA virus family Potyviridae.


ABSTRACT: The family Potyviridae encompasses ~30% of plant viruses and is responsible for significant economic losses worldwide. Recently, a small overlapping coding sequence, termed pipo, was found to be conserved in the genomes of all potyvirids. PIPO is expressed as part of a frameshift protein, P3N-PIPO, which is essential for virus cell-to-cell movement. However, the frameshift expression mechanism has hitherto remained unknown. Here, we demonstrate that transcriptional slippage, specific to the viral RNA polymerase, results in a population of transcripts with an additional "A" inserted within a highly conserved GAAAAAA sequence, thus enabling expression of P3N-PIPO. The slippage efficiency is ~2% in Turnip mosaic virus and slippage is inhibited by mutations in the GAAAAAA sequence. While utilization of transcriptional slippage is well known in negative-sense RNA viruses such as Ebola, mumps and measles, to our knowledge this is the first report of its widespread utilization for gene expression in positive-sense RNA viruses.

SUBMITTER: Olspert A 

PROVIDER: S-EPMC4552492 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Transcriptional slippage in the positive-sense RNA virus family Potyviridae.

Olspert Allan A   Chung Betty Y-W BY   Atkins John F JF   Carr John P JP   Firth Andrew E AE  

EMBO reports 20150625 8


The family Potyviridae encompasses ~30% of plant viruses and is responsible for significant economic losses worldwide. Recently, a small overlapping coding sequence, termed pipo, was found to be conserved in the genomes of all potyvirids. PIPO is expressed as part of a frameshift protein, P3N-PIPO, which is essential for virus cell-to-cell movement. However, the frameshift expression mechanism has hitherto remained unknown. Here, we demonstrate that transcriptional slippage, specific to the vira  ...[more]

Similar Datasets

| S-EPMC5027478 | biostudies-literature
| S-EPMC5861449 | biostudies-literature
| S-EPMC2567904 | biostudies-literature
| S-EPMC3400743 | biostudies-literature
| S-EPMC9618458 | biostudies-literature
| S-EPMC3831450 | biostudies-literature
| S-EPMC8006769 | biostudies-literature
| S-EPMC7251205 | biostudies-literature
| S-EPMC7737751 | biostudies-literature
| S-EPMC9093098 | biostudies-literature