Ontology highlight
ABSTRACT:
SUBMITTER: Wong S
PROVIDER: S-EPMC4552755 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Wong Stanley S Mosabbir Abdullah A AA Truong Kevin K
PloS one 20150828 8
Protein splicing is mediated by inteins that auto-catalytically join two separated protein fragments with a peptide bond. Here we engineered a genetically encoded synthetic photoactivatable intein (named LOVInC), by using the light-sensitive LOV2 domain from Avena sativa as a switch to modulate the splicing activity of the split DnaE intein from Nostoc punctiforme. Periodic blue light illumination of LOVInC induced protein splicing activity in mammalian cells. To demonstrate the broad applicabil ...[more]