Dataset Information

The Effect of Long-Term Continuous Cropping of Black Pepper on Soil Bacterial Communities as Determined by 454 Pyrosequencing.

ABSTRACT: In the present study, 3 replanted black pepper orchards with continuously cropping histories for 10, 21, and 55 years in tropical China, were selected for investigating the effect of monoculture on soil physiochemical properties, enzyme activities, bacterial abundance, and bacterial community structures. Results showed long-term continuous cropping led to a significant decline in soil pH, organic matter contents, enzymatic activities, and resulted in a decrease in soil bacterial abundance. 454 pyrosequencing analysis of 16S rRNA genes revealed that the Acidobacteria and Proteobacteria were the main phyla in the replanted black pepper orchard soils, comprising up to 73.82% of the total sequences; the relative abundances of Bacteroidetes and Firmicutes phyla decreased with long-term continuous cropping; and at genus level, the Pseudomonas abundance significantly depleted after 21 years continuous cropping. In addition, bacterial diversity significantly decreased after 55 years black pepper continuous cropping; obvious variations for community structures across the 3 time-scale replanted black pepper orchards were observed, suggesting monoculture duration was the major determinant for bacterial community structure. Overall, continuous cropping during black pepper cultivation led to a significant decline in soil pH, organic matter contents, enzymatic activities, resulted a decrease in soil bacterial abundance, and altered soil microbial community membership and structure, which in turn resulted in black pepper poor growth in the continuous cropping system.

SUBMITTER: Xiong W

PROVIDER: S-EPMC4552827 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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The Effect of Long-Term Continuous Cropping of Black Pepper on Soil Bacterial Communities as Determined by 454 Pyrosequencing.

Xiong Wu W Li Zhigang Z Liu Hongjun H Xue Chao C Zhang Ruifu R Wu Huasong H Li Rong R Shen Qirong Q

PloS one 20150828 8

In the present study, 3 replanted black pepper orchards with continuously cropping histories for 10, 21, and 55 years in tropical China, were selected for investigating the effect of monoculture on soil physiochemical properties, enzyme activities, bacterial abundance, and bacterial community structures. Results showed long-term continuous cropping led to a significant decline in soil pH, organic matter contents, enzymatic activities, and resulted in a decrease in soil bacterial abundance. 454 p ...[more]

PMID: 26317364

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Project description:Cover crops can improve soil biological health and alter the composition of soil microbial communities in agricultural systems. However, the effects of diversified cover crops on soil microbial communities in continuous cropping systems are unclear. Here, using different soil biochemical analysis, quantitative PCR and 16S rRNA amplicon sequencing, we investigated the effects of cover crops, alone or in mixture, on soil physicochemical properties in 2019 and 2020, and soil bacterial communities in 2020 in a continuous pepper cropping system. A field trial was established before pepper planting and eight treatments were included: fallow (no cover crop; CK); three sole cover crop treatments: wheat (Triticum aestivum L.; W), faba bean (Vicia faba L.; B), and wild rocket (Diplotaxis tenuifolia; R); and four mixed treatments: wheat + wild rocket (WR), wheat + faba bean (WB), wild rocket + faba bean (RB), and wheat + wild rocket + faba bean (WRB). The pepper yield was increased in the WR and WB in 2019 and 2020, and in the WRB in 2020. Cover crops increased the soil pH, but decreased the concentrations of NH4 + and available phosphorus. Bacterial abundance was increased by cover crop treatments, and community structure was altered in the W, WB, and WRB treatments. Moreover, we found that pH was the key factor associated with the changes in the abundance and structure of the bacterial community. Cover crop treatments altered the bacterial community structure with shifts in the dominant genera, which have plant-growth-promoting and/or pathogen-antagonistic potentials, e.g., increased the abundances of Streptomyces, Arthrobacter, and Bacillus spp. in the W and WRB, and Gaiella spp. in the WB. Overall, we found that cover crops altered the soil physicochemical properties and bacterial community, and these changes varied with species composition of the cover crops, with wheat and its combination with legumes as most effective treatments. These results suggest that the diversification within cover crops could provide better crop yield stimulatory affects by altering soil biochemical environment.

Project description:The continuous planting of soybeans leads to soil acidification, aggravation of soil-borne diseases, reduction in soil enzyme activity, and accumulation of toxins in the soil. Microorganisms in the rhizosphere play a very important role in maintaining the sustainability of the soil ecosystem and plant health. In this study, two soybean genotypes, one bred for continuous cropping and the other not, were grown in a Mollisol in northeast China under continuous cropping for 7 and 36years in comparison with soybean-maize rotation, and microbial communities in the rhizosphere composition were assessed using high-throughput sequencing technology. The results showed that short- or long-term continuous cropping had no significant effect on the rhizosphere soil bacterial alpha diversity. Short-term continuous planting increased the number of soybean cyst nematode (Heterodera glycines), while long-term continuous planting reduced these numbers. There were less soybean cyst nematodes in the rhizosphere of the tolerant genotypes than sensitive genotypes. In addition, continuous cropping significantly increased the potential beneficial bacterial populations, such as Pseudoxanthomonas, Nitrospira, and Streptomyces compared to rotation and short-term continuous cropping, suggesting that long-term continuous cropping of soybean shifts the microbial community toward a healthy crop rotation system. Soybean genotypes that are tolerant to soybean might recruit some microorganisms that enhance the resistance of soybeans to long-term continuous cropping. Moreover, the network of the two genotypes responded differently to continuous cropping. The tolerant genotype responded positively to continuous cropping, while for the sensitive genotype, topology analyses on the instability of microbial community in the rhizosphere suggested that short periods of continuous planting can have a detrimental effect on microbial community stability, although this effect could be alleviated with increasing periods of continuous planting.

Dataset Information

The Effect of Long-Term Continuous Cropping of Black Pepper on Soil Bacterial Communities as Determined by 454 Pyrosequencing.

Publications

The Effect of Long-Term Continuous Cropping of Black Pepper on Soil Bacterial Communities as Determined by 454 Pyrosequencing.

Similar Datasets

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