Diagnosis of Basal-Like Breast Cancer Using a FOXC1-Based Assay.

ABSTRACT: Diagnosis of basal-like breast cancer (BLBC) remains a bottleneck to conducting effective clinical trials for this aggressive subtype. We postulated that elevated expression of Forkhead Box transcription factor C1 (FOXC1) is a simple and accurate diagnostic biomarker for BLBC. Accuracy of FOXC1 expression in identifying BLBC was compared with the PAM50 gene expression panel in gene expression microarray (GEM) (n = 1992) and quantitative real-time polymerase chain reaction (qRT-PCR) (n = 349) datasets. A FOXC1-based immunohistochemical (IHC) assay was developed and assessed in 96 archival formalin-fixed, paraffin-embedded (FFPE) breast cancer samples that also underwent PAM50 profiling. All statistical tests were two-sided. A FOXC1-based two-tier assay (IHC +/- qRT-PCR) accurately identifie

SUBMITTER: Jensen TW 

PROVIDER: S-EPMC4554196 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature