Dataset Information

Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

ABSTRACT: Photoactivated localization microscopy (PALM) is a super-resolution imaging technique based on the detection and subsequent localization of single fluorescent molecules. PALM is therefore a powerful tool in resolving structures and putative interactions of biomolecules at the ultimate analytical detection limit. However, its limited imaging depth restricts PALM mostly to in vitro applications. Considering the additional need for anatomical context when imaging a multicellular organism, these limitations render the use of PALM in whole animals difficult. Here we integrated PALM with confocal microscopy for correlated imaging of the C. elegans nervous system, a technique we termed confocal correlated PALM (ccPALM). The neurons, lying below several tissue layers, could be visualized up to 10 ?m deep inside the animal. By ccPALM, we visualized ionotropic glutamate receptor distributions in C. elegans with an accuracy of 20 nm, revealing super-resolution structure of receptor clusters that we mapped onto annotated neurons in the animal. Pivotal to our results was the TIRF-independent detection of single molecules, achieved by genetic regulation of labeled receptor expression and localization to effectively reduce the background fluorescence. By correlating PALM with confocal microscopy, this platform enables dissecting biological structures with single molecule resolution in the physiologically relevant context of whole animals.

SUBMITTER: Vangindertael J

PROVIDER: S-EPMC4555104 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

Vangindertael Jeroen J Beets Isabel I Rocha Susana S Dedecker Peter P Schoofs Liliane L Vanhoorelbeke Karen K Hofkens Johan J Mizuno Hideaki H

Scientific reports 20150901

Photoactivated localization microscopy (PALM) is a super-resolution imaging technique based on the detection and subsequent localization of single fluorescent molecules. PALM is therefore a powerful tool in resolving structures and putative interactions of biomolecules at the ultimate analytical detection limit. However, its limited imaging depth restricts PALM mostly to in vitro applications. Considering the additional need for anatomical context when imaging a multicellular organism, these lim ...[more]

PMID: 26323790

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Dataset Information

Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

Publications

Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

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