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An Integrated Framework Advancing Membrane Protein Modeling and Design.

ABSTRACT: Membrane proteins are critical functional molecules in the human body, constituting more than 30% of open reading frames in the human genome. Unfortunately, a myriad of difficulties in overexpression and reconstitution into membrane mimetics severely limit our ability to determine their structures. Computational tools are therefore instrumental to membrane protein structure prediction, consequently increasing our understanding of membrane protein function and their role in disease. Here, we describe a general framework facilitating membrane protein modeling and design that combines the scientific principles for membrane protein modeling with the flexible software architecture of Rosetta3. This new framework, called RosettaMP, provides a general membrane representation that interfaces with scoring, conformational sampling, and mutation routines that can be easily combined to create new protocols. To demonstrate the capabilities of this implementation, we developed four proof-of-concept applications for (1) prediction of free energy changes upon mutation; (2) high-resolution structural refinement; (3) protein-protein docking; and (4) assembly of symmetric protein complexes, all in the membrane environment. Preliminary data show that these algorithms can produce meaningful scores and structures. The data also suggest needed improvements to both sampling routines and score functions. Importantly, the applications collectively demonstrate the potential of combining the flexible nature of RosettaMP with the power of Rosetta algorithms to facilitate membrane protein modeling and design.

SUBMITTER: Alford RF

PROVIDER: S-EPMC4556676 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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An Integrated Framework Advancing Membrane Protein Modeling and Design.

Alford Rebecca F RF Koehler Leman Julia J Weitzner Brian D BD Duran Amanda M AM Tilley Drew C DC Elazar Assaf A Gray Jeffrey J JJ

PLoS computational biology 20150901 9

Membrane proteins are critical functional molecules in the human body, constituting more than 30% of open reading frames in the human genome. Unfortunately, a myriad of difficulties in overexpression and reconstitution into membrane mimetics severely limit our ability to determine their structures. Computational tools are therefore instrumental to membrane protein structure prediction, consequently increasing our understanding of membrane protein function and their role in disease. Here, we desc ...[more]

PMID: 26325167

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Dataset Information

An Integrated Framework Advancing Membrane Protein Modeling and Design.

Publications

An Integrated Framework Advancing Membrane Protein Modeling and Design.

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