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Data from a proteomic baseline study of Assemblage A in Giardia duodenalis.


ABSTRACT: Eight Assemblage A strains from the protozoan parasite Giardia duodenalis were analysed using label-free quantitative shotgun proteomics, to evaluate inter- and intra-assemblage variation and complement available genetic and transcriptomic data. Isolates were grown in biological triplicate in axenic culture, and protein extracts were subjected to in-solution digest and online fractionation using Gas Phase Fractionation (GPF). Recent reclassification of genome databases for subassemblages was evaluated for database-dependent loss of information, and proteome composition of different isolates was analysed for biologically relevant assemblage-independent variation. The data from this study are related to the research article "Quantitative proteomics analysis of Giardia duodenalis Assemblage A - a baseline for host, assemblage and isolate variation" published in Proteomics (Emery et al., 2015 [1]).

SUBMITTER: Emery SJ 

PROVIDER: S-EPMC4556777 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Data from a proteomic baseline study of Assemblage A in Giardia duodenalis.

Emery Samantha J SJ   Lacey Ernest E   Haynes Paul A PA  

Data in brief 20150819


Eight Assemblage A strains from the protozoan parasite Giardia duodenalis were analysed using label-free quantitative shotgun proteomics, to evaluate inter- and intra-assemblage variation and complement available genetic and transcriptomic data. Isolates were grown in biological triplicate in axenic culture, and protein extracts were subjected to in-solution digest and online fractionation using Gas Phase Fractionation (GPF). Recent reclassification of genome databases for subassemblages was eva  ...[more]

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