Ontology highlight
ABSTRACT:
SUBMITTER: Subburaj Y
PROVIDER: S-EPMC4557355 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Subburaj Yamunadevi Y Cosentino Katia K Axmann Markus M Pedrueza-Villalmanzo Esteban E Hermann Eduard E Bleicken Stephanie S Spatz Joachim J García-Sáez Ana J AJ
Nature communications 20150814
Bax is a key regulator of apoptosis that mediates the release of cytochrome c to the cytosol via oligomerization in the outer mitochondrial membrane before pore formation. However, the molecular mechanism of Bax assembly and regulation by other Bcl-2 members remains obscure. Here, by analysing the stoichiometry of Bax oligomers at the single-molecule level, we find that Bax binds to the membrane in a monomeric state and then self-assembles in <1 min. Strikingly, active Bax does not exist in a un ...[more]