Kinetic fingerprinting to identify and count single nucleic acids.
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ABSTRACT:
SUBMITTER: Johnson-Buck A
PROVIDER: S-EPMC4559481 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Kinetic fingerprinting to identify and count single nucleic acids.
Johnson-Buck Alexander A Su Xin X Giraldez Maria D MD Zhao Meiping M Tewari Muneesh M Walter Nils G NG
Nature biotechnology 20150622 7
MicroRNAs (miRNAs) have emerged as promising diagnostic biomarkers. We introduce a kinetic fingerprinting approach called single-molecule recognition through equilibrium Poisson sampling (SiMREPS) for the amplification-free counting of single unlabeled miRNA molecules, which circumvents thermodynamic limits of specificity and virtually eliminates false positives. We demonstrate high-confidence, single-molecule detection of synthetic and endogenous miRNAs in both buffer and minimally treated biof ...[more]
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