Deep Sequence Analysis of AgoshRNA Processing Reveals 3' A Addition and Trimming.
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ABSTRACT:
SUBMITTER: Harwig A
PROVIDER: S-EPMC4561654 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Deep Sequence Analysis of AgoshRNA Processing Reveals 3' A Addition and Trimming.
Harwig Alex A Herrera-Carrillo Elena E Jongejan Aldo A van Kampen Antonius Hubertus AH Berkhout Ben B
Molecular therapy. Nucleic acids 20150714
The RNA interference (RNAi) pathway, in which microprocessor and Dicer collaborate to process microRNAs (miRNA), was recently expanded by the description of alternative processing routes. In one of these noncanonical pathways, Dicer action is replaced by the Argonaute2 (Ago2) slicer function. It was recently shown that the stem-length of precursor-miRNA or short hairpin RNA (shRNA) molecules is a major determinant for Dicer versus Ago2 processing. Here we present the results of a deep sequence s ...[more]
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