Novel high-throughput deoxyribonuclease 1 assay.
Ontology highlight
ABSTRACT:
SUBMITTER: Jang DS
PROVIDER: S-EPMC4564109 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Publications
Novel high-throughput deoxyribonuclease 1 assay.
Jang Dae Song DS Penthala Narsimha R NR Apostolov Eugene O EO Wang Xiaoying X Fahmi Tariq T Crooks Peter A PA Basnakian Alexei G AG
Journal of biomolecular screening 20141017 2
Deoxyribonuclease I (DNase I), the most active and abundant apoptotic endonuclease in mammals, is known to mediate toxic, hypoxic, and radiation injuries to the cell. Neither inhibitors of DNase I nor high-throughput methods for screening of high-volume chemical libraries in search of DNase I inhibitors are, however, available. To overcome this problem, we developed a high-throughput DNase I assay. The assay is optimized for a 96-well plate format and based on the increase of fluorescence intens ...[more]
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