Ontology highlight
ABSTRACT:
SUBMITTER: Zandarashvili L
PROVIDER: S-EPMC4564683 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Biophysical journal 20150901 5
Dithioation of DNA phosphate is known to enhance binding affinities, at least for some proteins. We mechanistically characterized this phenomenon for the Antennapedia homeodomain-DNA complex by integrated use of fluorescence, isothermal titration calorimetry, NMR spectroscopy, and x-ray crystallography. By fluorescence and isothermal titration calorimetry, we found that this affinity enhancement is entropy driven. By NMR, we investigated the ionic hydrogen bonds and internal motions of lysine si ...[more]