Ontology highlight
ABSTRACT:
SUBMITTER: Burns S
PROVIDER: S-EPMC4564689 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
eLife 20150915
Duplication of the yeast centrosome (called the spindle pole body, SPB) is thought to occur through a series of discrete steps that culminate in insertion of the new SPB into the nuclear envelope (NE). To better understand this process, we developed a novel two-color structured illumination microscopy with single-particle averaging (SPA-SIM) approach to study the localization of all 18 SPB components during duplication using endogenously expressed fluorescent protein derivatives. The increased r ...[more]