Ontology highlight
ABSTRACT:
SUBMITTER: Hayashi A
PROVIDER: S-EPMC4566276 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Hayashi Azusa A Mochizuki Kazufumi K
Genetics 20150723 1
Tetrahymena is a useful eukaryotic model for biochemistry and molecular cell biology studies. We previously demonstrated that targeted ectopic DNA elimination, also called co-Deletion (coDel), can be induced by the introduction of an internal eliminated sequence (IES)-target DNA chimeric construct. In this study, we demonstrate that coDel occurs at most of the loci tested and can be used for the production of somatic gene KO strains. We also showed that coDel at two loci can be simultaneously in ...[more]