Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC4567307 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Liu Yun Y Dun Baoqing B Shi Pengjun P Ma Rui R Luo Huiying H Bai Yingguo Y Xie Xiangming X Yao Bin B
PloS one 20150911 9
An endo-β-1,4-glucanase gene, cel7A, was cloned from the thermophilic cellulase-producing fungus Neosartorya fischeri P1 and expressed in Pichia pastoris. The 1,410-bp full-length gene encodes a polypeptide of 469 amino acids consisting of a putative signal peptide at residues 1-20, a catalytic domain of glycoside hydrolase family 7 (GH7), a short Thr/Ser-rich linker and a family 1 carbohydrate-binding module (CBM 1). The purified recombinant Cel7A had pH and temperature optima of pH 5.0 and 60° ...[more]