Ontology highlight
ABSTRACT:
SUBMITTER: Tian N
PROVIDER: S-EPMC4568467 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Scientific reports 20150914
We extend the subtractive imaging method to label-free second harmonic generation (SHG) microscopy to enhance the spatial resolution and contrast. This method is based on the intensity difference between two images obtained with circularly polarized Gaussian and doughnut-shaped beams, respectively. By characterizing the intensity and polarization distributions of the two focused beams, we verify the feasibility of the subtractive imaging method in polarization dependent SHG microscopy. The resol ...[more]