Hyperspectral light sheet microscopy.
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ABSTRACT:
SUBMITTER: Jahr W
PROVIDER: S-EPMC4569691 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Publications
Hyperspectral light sheet microscopy.
Jahr Wiebke W Schmid Benjamin B Schmied Christopher C Fahrbach Florian O FO Huisken Jan J
Nature communications 20150902
To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varying spectral sampling and determined the optimal channel width to be around 5 nm. With the help of these ...[more]
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